名称：抗Visfatin抗体(anti-Visfatin antibody)

　　货号：FNab09847

　　适应种属：人源，大鼠，小鼠

　　规格：100ug(微克)

　　状态：液体

　　纯化方法：蛋白A/G柱纯化

　　纯度：经sds-page分析，纯度大于95

　　宿主：小鼠

　　亚型：IgG2b

　　存储：用添加了0.02叠氮化钠和50甘油的pbs溶解，ph值7.3，可在-20℃下保存12个月（避免反复冻融）

　　免疫原：nicotinamide phosphoribosyltransferase

　　免疫原别称：1110035O14Rik, DKFZp666B131, NAmPRTase, NAMPT, PBEF, PBEF1, Pre B cell enhancing factor, VF, VISFATIN

　　分子量：52 kDa

　　验证应用：ELISA, WB, IHC, IF

　　建议稀释比例：WB: 1:1000-1:4000; IHC: 1:50-1:500; IF: 1:50-1:500

　　克隆性：单克隆

　　uniprot id：P43490

　　背景介绍：Nicotinamide phosphoribosyltransferase (NAMPT) has two usual synonyms termed Visfatin and PBEF. Its primary role is to catalyze the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD, which is the rate limiting component in the mammalian NAD biosynthesis pathway. NAMPT is localized in cytoplasm and expressed in large amounts in bone marrow, liver tissue, and muscle tissues. NAMPT inhibits neutrophil apoptosis in experimental inflammation and clinical sepsis. NAMPT levels are altered in plasma of patients with type 2 diabetes mellitus (T2DM) , and it is now evidenced that NAMPT may plays a role in lipid bbolism。

　　Immunohistochemistry of paraffin-bded human breast cancer tissue slide using FNab09847 (Visfatin antibody) at dilution of 1:200。

　　Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using FNab09847( Visfatin antibody ) at dilution of 1:100 and Alexa Fluor 488-congugated Goat Anti-Mouse IgG(H L)。

　　rat heart tissue were subjected to SDS PAGE followed by western blot with FNab09847 (Visfatin antibody) at dilution of 1:2000。